My current mains in WoW are male -- but I have been harassed on Vent due to my gender as soon as it was known.
PBEMs, I play both genders equally. Since I want to be a writer, I figure it's good practice.
E-wrestling, I play a majority of male characters. Back when I started in E-W, I was TERRIFIED of my real gender being known because in that particular circle, some female players had been harassed and attacked before. One notorious fed had
(spoilers for NSFW bit)
And the handlers just laughed about it. Needless to say, I avoided that place and those handlers.